Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Role of mTOR signaling in activating TME cells. This image illustrates the activation of TME cells via mTOR signaling pathway emphasising importance of mTOR in cancer progression and development. a it depicts that growth factors or high energy levels involve in dimerization of RTK receptor in-turn recruit PI3K thereby phosphorylating PIP2 to PIP3. This results in the activation of AKT by PDK1. It promotes function of mTORC1. AKT also negatively modulated by TSC with phosphorylation of RHEB-GDP to RHEB-GTP. Activated mTOR in TME initiates expression of PD-L1 in T cells which causes decrease in T-cell based immune function in cancer cells. Additionally, mTOR also promotes differentiation of T cells to Tregs leading to immunosuppression and cancer progression. b Receptor-ligand binding occur in CAF, recruits PI3K which can promote cascade of PIP2 to PIP3 thereby activating mTOR by PDK1/AKT. mTOR is then transferred to nuclei following with differentiation of CAF. CAF derived chemokines and cytokines binds to the receptor in cancer cell promoting PI3K/AKT/mTOR cascade which regulates cell proliferation, progression of cancer. c TME is mainly composed of M2 type of macrophages. Polarization of M1 and M2 have distinct activating mechanism where this depicts only M2 mechanism. Polarization of M2 occurs through binding of Type I- IL-4 to JAK/STAT receptor thereby activating IRSs which cannot be translocated to nucleus induces PI3K/AKT/mTOR pathway and trigger gene expression that promotes macrophage activation. In contrast STAT3 can also be produced which has ability to translocate itself into nucleus and induce macrophage activation ( www.biorender.com )

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Activation Assay, Expressing, Ligand Binding Assay, Derivative Assay, Binding Assay, Produced

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Positive controls used for IHC Analysis

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Positive Control

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Survival graphs based on high and low expression of markers ( A ) CD163, ( B ) α-SMA, ( C ) mTOR, ( D ) FOXp3

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Expressing

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Two marker combination survival analysis (Fig. 6 )

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Marker, Expressing

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Three marker combination survival analysis (Fig. 7 )

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Marker, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Dihydroartemisinin inhibits the activation and proliferation of hepatic stellate cells by regulating miR-29b-3p

doi: 10.3892/ijmm.2023.5243

Figure Lengend Snippet: VEGFR1 and VEGFR2 have different roles in hepatic stellate cell activation. (A) Wound healing assay of LX-2 cells transfected with VEGFA siRNA, VEGFR1 siRNA and VEGR2 siRNA. (B) Adhesion assay of LX-2 cells transfected with VEGFA siRNA, VEGFR1 siRNA and VEGR2 siRNA. (C) Annexin V-FITC/PI double staining of LX-2 cells transfected with VEGFA siRNA, VEGFR1 siRNA and VEGR2 siRNA. (D) ELISA analysis of IL-6 in LX-2 cells transfected with VEGFA siRNA, VEGFR1 siRNA and VEGR2 siRNA. (E) Western blot analysis of Bax/Bcl-2, p62 and LC3-II/LC3-I in LX-2 cells transfected with VEGFR1 siRNA and VEGFR2 siRNA. (F) Western blot analysis of Bax/Bcl-2, p62 and LC3-I/LC3-II in LX-2 cells treated with GNQWFI and SU5416. Data are expressed as the mean ± SD (n=3). * P<0.05, ** P<0.01, *** P<0.001. siRNA/si, small interfering RNA.

Article Snippet: The primary antibodies against VEGFR1 (cat. no. A19132), AKT (cat. no. A20799), phosphorylated (p)-AKT (cat. no. AP1259), mTOR (cat. no. A2445), p-mTOR (cat. no. AP0115), ULK1 (cat. no. A8529), p-ULK1 (cat. no. AP0736), caspase-9 (cat. no. A18676), cytochrome c (cat. no. A4912), AIF (cat. no. A19536), LC3B (cat. no. A19665), Beclin-1 (cat. no. A7353) and P62 (cat. no. A19700) were purchased from ABclonal Biotech Co., Ltd.

Techniques: Activation Assay, Wound Healing Assay, Transfection, Cell Adhesion Assay, Double Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Small Interfering RNA

Journal: Experimental neurology

Article Title: Impaired Social Behaviors and Minimized Oxytocin Signaling of the Adult Mice Deficient in the N-Methyl-D-Aspartate Receptor GluN3A Subunit

doi: 10.1016/j.expneurol.2018.02.015

Figure Lengend Snippet: Western blot analysis was performed on brain tissues from the hippocampus and thalamus areas of WT and GluN3A KO mice. A. Western blot images and quantification of the band intensity compared to the basal level. The protein level of oxytocin receptor, serotonin receptors 5-HTR2A, 5-HTR1B, TNFR1 and CD73 showed no statistically difference between WT and KO mice. The level of CD38, however, was significantly higher in the KO hippocampus. N=6, Student t test; * p<0.05, F=1.299. B. The above assay was repeated in the thalamus. All measured protein expressions were similar between WT and KO mice, expect the 5-HTR2A level was significantly lower in this brain region. N=6; Student t test; * p<0.05, F=5.650.

Article Snippet: The primary antibodies used and the dilutions for each were mouse anti-serotonin receptor (5-HTR; BD Bioscience, San Jose, CA, USA) at 1:2,500, rabbit anti-serotonin transporter (BD Bioscience) at 1:1,000, rabbit anti-oxytocin antibody (Fitzgerald Industries International, North Acton, MA, USA) at 1:1000, goat anti-oxytocin receptor antibody (Abcam, Cambridge, MA, USA) at 1:2,000, goat anti-CD73 antibody (Santa Cruz Biotech, Inc., Dallas, TX, USA) at 1:2,000, rabbit anti-BDNF antibody (Santa Cruz Biotech) at 1:2,000, mouse anti-TNFR1 antibody (Santa Cruz Biotech) at 1:2,000, rabbit anti-GluN3A antibody (Abcam) at 1:2,000, and mouse anti-actin (Sigma-Aldrich).

Techniques: Western Blot

Journal: Experimental neurology

Article Title: Impaired Social Behaviors and Minimized Oxytocin Signaling of the Adult Mice Deficient in the N-Methyl-D-Aspartate Receptor GluN3A Subunit

doi: 10.1016/j.expneurol.2018.02.015

Figure Lengend Snippet: We investigated the possibility that the deletion of GluN3A could altered social behavior-associated genes such as serotonin receptor (5-HTR), serotonin transporter (5-HTT), BDNF, CD73, and TNFR1. A. Western blotting of serotonin receptor, serotonin transporter, BDNF, CD73, and TNFR1 in the prefrontal cortex from WT and GluN3A KO mice. B-F. Quantification of the optic density (normalized to β-actin control) of 5-HTR, 5-HTT, BDNF, CD73, and TNFR1 in the prefrontal cortex. The expression of these genes was also unchanged in GluN3A KO mice. N=6 per group.

Article Snippet: The primary antibodies used and the dilutions for each were mouse anti-serotonin receptor (5-HTR; BD Bioscience, San Jose, CA, USA) at 1:2,500, rabbit anti-serotonin transporter (BD Bioscience) at 1:1,000, rabbit anti-oxytocin antibody (Fitzgerald Industries International, North Acton, MA, USA) at 1:1000, goat anti-oxytocin receptor antibody (Abcam, Cambridge, MA, USA) at 1:2,000, goat anti-CD73 antibody (Santa Cruz Biotech, Inc., Dallas, TX, USA) at 1:2,000, rabbit anti-BDNF antibody (Santa Cruz Biotech) at 1:2,000, mouse anti-TNFR1 antibody (Santa Cruz Biotech) at 1:2,000, rabbit anti-GluN3A antibody (Abcam) at 1:2,000, and mouse anti-actin (Sigma-Aldrich).

Techniques: Western Blot, Expressing

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases

doi: 10.1152/ajpgi.00145.2019

Figure Lengend Snippet: Molecular markers and proteins associated with metastases. A: Western blot of total protein from tumor samples from mice treated with PBS, polyclonal antibody stimulator (PAS), programmed cell death receptor (PD)-1 Ab, and PAS/PD-1 Ab combination probed with antibody specific for Tyr118 phosphorylated paxillin (top), total paxillin (middle), and β-actin (bottom). B: ratio of phosphorylated paxillin relative to β-actin plotted for each treatment group (*P = 0.01, compared with PBS). C: change in E-cadherin mRNA in tumors of PAS-treated mice relative to PBS control. D: change in tumor necrosis factor-β receptor 2 (TGF-BR2) mRNA in tumors of PAS-treated mice relative to PBS control. E: Western blot of total protein from tumor samples probed with β-catenin and β-actin. L, ladder. F: levels of β-catenin protein relative to β-actin in tumors of treated mice. G: Western blot of total protein from tumor samples probed with mouse matrix metalloproteinase-7 (MMP-7) and β-actin. Lad, ladder. H: levels of MMP-7 protein relative to β-actin in tumor samples (see Graphical Abstract). Effect of PAS on epithelial-mesenchymal transition (EMT) and tumor metastases. Gastrin stimulates growth and metastasis of pancreatic cancer through activation of the cholecystokinin (CCK-BR) receptor. In tumors of PBS-treated mice, the tumor microenvironment has extensive fibrosis and M2 tumor associated macrophages. The macrophages release CxCR4 to activate TGF-β signaling. The cancer epithelial cells undergo EMT through a metastatic cascade process that involves disruption in the adherens junctions associated with loss of E-cadherin. Gastrin activates phosphorylation of the focal adhesion protein paxillin and activation of the MMP-7 promoter increasing β-catenin expression and nuclear translocation leading to cell migration. Vaccination with PAS interrupts the action of gastrin leading to decreased fibrosis of the tumor microenvironment and reversing EMT, thus decreasing metastases. *Significant compared with PBS.

Article Snippet: This blot was probed overnight at 4°C with a primary monoclonal murine antibody to β-catenin (cat. no. 610154, lot: 25190; BD Transduction Laboratories) at a dilution of 1:2,000.

Techniques: Western Blot, Activation Assay, Expressing, Translocation Assay, Migration

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Positive controls used for IHC Analysis

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Positive Control

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Survival graphs based on high and low expression of markers ( A ) CD163, ( B ) α-SMA, ( C ) mTOR, ( D ) FOXp3

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Expressing

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Two marker combination survival analysis (Fig. 6 )

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Marker, Expressing

Journal: BMC Oral Health

Article Title: Expression of mTOR, CD163, α-SMA, FOXp3 as survival predictors and its significance in patients with oral squamous cell carcinoma

doi: 10.1186/s12903-024-05245-y

Figure Lengend Snippet: Three marker combination survival analysis (Fig. 7 )

Article Snippet: It was followed by incubating the sections with primary monoclonal antibodies targeting FoxP3 + T-cells (prediluted; clone 236 A/E7; Lot: 02122 A; BIOCARE, USA), CD163 + Macrophages (prediluted; clone EP324; Lot:6305FME18; BIOSB, USA), mTOR of tumor cells (prediluted; clone 59.Ser 2448; Lot: MC0509R0622; MEDAYSIS, USA), α-SMA of Cancer associated fibroblasts (prediluted; clone 1A4; Lot:1105092D-3 F; ZETA Corporation, USA) at room temperature overnight.

Techniques: Marker, Expressing

Journal: Journal of Inflammation (London, England)

Article Title: Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis

doi: 10.1186/1476-9255-9-47

Figure Lengend Snippet: Forward and reverse primer sequences, RT-PCR product length and accession number (Acc.No.) in the EMBL database

Article Snippet: The membranes were then incubated overnight at 4°C with a 1:200 dilution of an anti-iNOS (610332, BD Transduction) rabbit polyclonal antibody in PBS-T20 with 3% milk powder or at 4°C overnight with a 1:500 dilution of an anti-eNOS mouse monoclonal antibody (Sa-258, Biomol, Butler Pike, Plymouth Meeting, PA, USA) in Tris Buffered Saline-T20 (TBS-T20 20 mM Tris–HCl, pH 7.4, 500 mM NaCl, 0.1% T-20).

Techniques: Sequencing

Journal: Journal of Inflammation (London, England)

Article Title: Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis

doi: 10.1186/1476-9255-9-47

Figure Lengend Snippet: Relative gene expression of iNOS and eNOS in pAECs under standard culture conditions at different time periods. Relative mRNA data are expressed as delta Ct (HPRT Ct - iNOS or eNOS Ct). The data represent the mean ± SEM (n = 3). Time of culture did not influence iNOS and eNOS mRNA expression.

Article Snippet: The membranes were then incubated overnight at 4°C with a 1:200 dilution of an anti-iNOS (610332, BD Transduction) rabbit polyclonal antibody in PBS-T20 with 3% milk powder or at 4°C overnight with a 1:500 dilution of an anti-eNOS mouse monoclonal antibody (Sa-258, Biomol, Butler Pike, Plymouth Meeting, PA, USA) in Tris Buffered Saline-T20 (TBS-T20 20 mM Tris–HCl, pH 7.4, 500 mM NaCl, 0.1% T-20).

Techniques: Gene Expression, Expressing

Journal: Journal of Inflammation (London, England)

Article Title: Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis

doi: 10.1186/1476-9255-9-47

Figure Lengend Snippet: Relative gene expression of iNOS (A) and eNOS (B) in pAECs treated with LPS (10 μg/ml) at different time periods (n = 3). LPS increased iNOS mRNA expression while eNOS mRNA expression was decreased. Relative mRNA data are expressed as the fold of increase (ΔΔCt method) in respect to the control (CTR = mean ± SEM of all control time points). Error bars represent the range of relative expression. The different letters above the bars indicate significant differences in the various time points (p < 0.05, ANOVA post hoc Tukey’s test).

Article Snippet: The membranes were then incubated overnight at 4°C with a 1:200 dilution of an anti-iNOS (610332, BD Transduction) rabbit polyclonal antibody in PBS-T20 with 3% milk powder or at 4°C overnight with a 1:500 dilution of an anti-eNOS mouse monoclonal antibody (Sa-258, Biomol, Butler Pike, Plymouth Meeting, PA, USA) in Tris Buffered Saline-T20 (TBS-T20 20 mM Tris–HCl, pH 7.4, 500 mM NaCl, 0.1% T-20).

Techniques: Gene Expression, Expressing, Control

Journal: Journal of Inflammation (London, England)

Article Title: Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis

doi: 10.1186/1476-9255-9-47

Figure Lengend Snippet: Expression of eNOS protein in pAECs treated with LPS (10 μg/ml) for different time periods (7, 15, 24 h). A ) LPS induced a significant decrease of eNOS protein expression in respect to the control (CTR = mean ± SEM of all control time points). The data represent the mean ± SEM (n = 3) of the relative protein content (AU = Arbitrary Units). The different letters above the bars indicate significant differences among the various time points (p < 0.05, ANOVA post hoc Tukey’s test, n = 3). B ) Representative Western Blot of eNOS and relative housekeeping β-tubulin were reported.

Article Snippet: The membranes were then incubated overnight at 4°C with a 1:200 dilution of an anti-iNOS (610332, BD Transduction) rabbit polyclonal antibody in PBS-T20 with 3% milk powder or at 4°C overnight with a 1:500 dilution of an anti-eNOS mouse monoclonal antibody (Sa-258, Biomol, Butler Pike, Plymouth Meeting, PA, USA) in Tris Buffered Saline-T20 (TBS-T20 20 mM Tris–HCl, pH 7.4, 500 mM NaCl, 0.1% T-20).

Techniques: Expressing, Control, Western Blot

Journal: Journal of Inflammation (London, England)

Article Title: Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis

doi: 10.1186/1476-9255-9-47

Figure Lengend Snippet: Representative eNOS immunofluorescent staining on pAEC. Green fluorescence indicates positive immunostaining revealed by FITC, red fluorescence indicates nuclear staining with Propidium Iodide (magnification 400X). A , B ) eNOS protein localisation in standard cultured pAECs; a cytoplasmatic signal evenly diffused was detected. ( C - F ) LPS treatment resulted in a modification of the signal with more intense staining in the perinuclear region.

Article Snippet: The membranes were then incubated overnight at 4°C with a 1:200 dilution of an anti-iNOS (610332, BD Transduction) rabbit polyclonal antibody in PBS-T20 with 3% milk powder or at 4°C overnight with a 1:500 dilution of an anti-eNOS mouse monoclonal antibody (Sa-258, Biomol, Butler Pike, Plymouth Meeting, PA, USA) in Tris Buffered Saline-T20 (TBS-T20 20 mM Tris–HCl, pH 7.4, 500 mM NaCl, 0.1% T-20).

Techniques: Staining, Fluorescence, Immunostaining, Cell Culture, Modification

Journal: International Journal of Molecular Sciences

Article Title: Lgr5 Does Not Vary Throughout the Menstrual Cycle in Endometriotic Human Eutopic Endometrium

doi: 10.3390/ijms20010022

Figure Lengend Snippet: Immunofluorescence measurement of LGR5 expression throughout the menstrual cycle in eutopic endometrium. ( A ) Epithelial and stromal expression of LGR5 in control and endometriosis tissue in five phases of the menstrual cycle. ( B ) Total tissue expression across five phases of the menstrual cycle in control and endometriosis tissues. ( C ) Differences in LGR5 expression throughout the menstrual cycle between control and endometriosis tissue groups ( t -test for each phase; proliferative: p = 0.0242; secretory: p = 0.0424; menstruation: p = 0.0121). (Control: n = 24; endometriosis: n = 24). ( D ) Example of immunofluorescence of LGR5 in eutopic endometrium (early secretory phase). In blue: DAPI; in red: LGR5. * p < 0.05.

Article Snippet: Samples stained with monoclonal rabbit anti-LGR5 primary antibody (1 μL per million cells was used; BioNova Scientific, Fremont, CA, USA) and goat Alexa647 anti-rabbit secondary antibody (Invitrogen) at a 1:500 dilution.

Techniques: Immunofluorescence, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Lgr5 Does Not Vary Throughout the Menstrual Cycle in Endometriotic Human Eutopic Endometrium

doi: 10.3390/ijms20010022

Figure Lengend Snippet: In vitro analysis of LGR5 expression throughout the menstrual cycle in endometrial stromal fibroblast primary culture after treatment (E 2 and E 2 P 4 for six and six more days, respectively). ( A ) Left panels show expression of CYR61 (marker of the proliferative phase) and DKK1 (marker of the secretory phase) in the control group ( n = 4); right panels show expression of CYR61 and DKK1 in the endometriosis group ( n = 3). ( B ) Upper panel shows expression of LGR5 in proliferative (E 2 ) and secretory (E 2 P 4 ) phases of the control group; lower panel shows LGR5 expression in both phases of the endometriosis group (E 2 : estradiol; P 4 : progesterone).

Article Snippet: Samples stained with monoclonal rabbit anti-LGR5 primary antibody (1 μL per million cells was used; BioNova Scientific, Fremont, CA, USA) and goat Alexa647 anti-rabbit secondary antibody (Invitrogen) at a 1:500 dilution.

Techniques: In Vitro, Expressing, Marker, Control

Journal: International Journal of Molecular Sciences

Article Title: Lgr5 Does Not Vary Throughout the Menstrual Cycle in Endometriotic Human Eutopic Endometrium

doi: 10.3390/ijms20010022

Figure Lengend Snippet: Percentage of FACS-sorted LGR5 + cells. ( A ) Percentages of LGR5 + cells in different phases of the cycle (proliferative: n = 9; secretory: n = 4; ovulatory: n = 12; ND: n = 9). ( B ) Percentage of LGR5 + cells in eutopic endometrium of different types of endometriosis and controls (ovarian: n = 8; pelvic: n = 3; adenomyosis: n = 4; DIE: n = 10; control: n = 12). ( C ) Percentage of LGR5 + cells identified in eutopic endometrium of women with and without endometriosis (control: n = 12; endometriosis: n = 25). ( D ) Differences in LGR5 + cells between eutopic and ectopic endometrium. Left panel: ovarian endometriosis (eutopic: n = 8; ectopic: n = 4; p = 0.0286). Right panel: DIE (eutopic: n = 10; ectopic: n = 6; p = 0.0411). ( E ) Difference between ovarian and DIE ectopic endometrium (ovarian: n = 4; DIE: n = 6; p = 0.0381). ND: non-determined; DIE: deep infiltrating endometriosis. * p < 0.05, ** p < 0.03.

Article Snippet: Samples stained with monoclonal rabbit anti-LGR5 primary antibody (1 μL per million cells was used; BioNova Scientific, Fremont, CA, USA) and goat Alexa647 anti-rabbit secondary antibody (Invitrogen) at a 1:500 dilution.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Lgr5 Does Not Vary Throughout the Menstrual Cycle in Endometriotic Human Eutopic Endometrium

doi: 10.3390/ijms20010022

Figure Lengend Snippet: Primers used for RT-qPCR.

Article Snippet: Samples stained with monoclonal rabbit anti-LGR5 primary antibody (1 μL per million cells was used; BioNova Scientific, Fremont, CA, USA) and goat Alexa647 anti-rabbit secondary antibody (Invitrogen) at a 1:500 dilution.

Techniques: Sequencing